Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis
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چکیده
منابع مشابه
Detection of Leishmania DNA in paraffin embedded specimens of chronic lupoid leishmaniasis using polymerase chain reaction
Background: Chronic lupoid leishmaniasis (CLL) is a chronicform of cutaneous leishmaniasis that is usually resistant to antileishmania agents and leishmania is not seen in the histologicsections. We used polymerase chain reaction (PCR) to detectleishmania DNA in CLL specimens.Method: This descriptive cross sectional study was done on20 paraffin embedded specimens of CLL cases referred to theder...
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Polymerase chain reaction (PCR)-based detection of New World Leishmania from different types of clinical specimens has been further streamlined for field use by simplifying sample preparation and modifying published protocols. A multiplex PCR reaction was developed that allows simultaneous detection of the Leishmania genus and identification of the L. braziliensis complex. For typing isolates i...
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Background: Geographical distribution of zoonotic cutaneous leishmaniasis (ZCL) has continuously been extended in recent years in Iran. The Beiza District is one of the newly-emerged endemic foci of ZCL in southern Iran. The main aim of the present study was to detect the vector(s) of ZCL in this area. Methods: To detect the fauna and vectors of ZCL in this district, sand flies were cau...
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Cutaneous leishmaniasis (CL) can occur in skin and mucosa, causing disfiguring lesions. The laboratory diagnosis of CL involves immunological methods and optical detection of the parasite, al of which have limitations. There is a need for more effective diagnostic methods for CL which wil allow treatment to be initiated more promptly in order to help prevent the development of severe forms of m...
متن کاملComparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis: diagnosis of cutaneous leishmaniasis by polymerase chain reaction.
An evaluation of 5 laboratory methods for diagnosing American cutaneous leishmaniasis (ACL) was carried out on patients from an endemic area of Brazil. From 164 patients presenting cutaneous lesions, and suspected to have ACL, 133 (81.1%) were confirmed for the disease by Montenegro skin test (MST) and/or parasitologic examination (PE). In both groups of patients, the positivity of polymerase c...
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ژورنال
عنوان ژورنال: The American Journal of Tropical Medicine and Hygiene
سال: 2011
ISSN: 0002-9637,1476-1645
DOI: 10.4269/ajtmh.2011.10-0556